JOINT SUMMER SCHOOL

- PRACTICAL PART

Participants – undergraduate students from the Comenius University in Bratislava, Faculty of Natural Sciences

At the beginning.......

Session I: Microfluidic system and advanced in vitro models

Microfluidic system for cell cultivation on
transwells

Microfluidic cell-on-the-chip
cultivation system

2D cell cultivation

organoids (3D) cell cultivation

The undergraduate students visited the animal facility at the BMC SAV.

Session II: Basic and translational research

Preparation of cells for cell cycle analysis by flow cytometry.

BD FACSCanto II flow cytometer A. Distribution of cells. B. Cell cycle histogram

Cell cycle analysis by flow cytometry

Introduction to immunocytochemistry and cell seeding on coverslips

Analysis of cytoskeleton of mouse podocytes by immunocytochemistry
(Blue- nucleus, red- actin fibers, green-vinculin, 60x magnification)

Sample preparation for immunohistochemistry

Session III: Clinical oncology research

Preparation of patient samples for ddPCR

Droplet generator Droplet reader

Sample results from patient UM56. Black = no template control, green = negative control (wild type), blue = positive control (primary uveal melanoma cells), orange = metastatic uveal melanoma cells

Session IV: Nanosafety research in regulatory context: Challenges and opportunities

Preparation of solutions for the comet assay and insertion of the samples into the electrophoretic solution

.....at the end.