JOINT SUMMER SCHOOL
- PRACTICAL PART
Participants – undergraduate students from the Comenius University in Bratislava, Faculty of Natural Sciences
At the beginning.......
Session I: Microfluidic system and advanced in vitro models
| Microfluidic system for cell cultivation on
| Microfluidic cell-on-the-chip
| 2D cell cultivation
|| organoids (3D) cell cultivation
The undergraduate students visited the animal facility at the BMC SAV.
Session II: Basic and translational research
Preparation of cells for cell cycle analysis by flow cytometry.
BD FACSCanto II flow cytometer A. Distribution of cells. B. Cell cycle histogram
Cell cycle analysis by flow cytometry
Introduction to immunocytochemistry and cell seeding on coverslips
Analysis of cytoskeleton of mouse podocytes by immunocytochemistry
(Blue- nucleus, red- actin fibers, green-vinculin, 60x magnification)
Sample preparation for immunohistochemistry
Session III: Clinical oncology research
Preparation of patient samples for ddPCR
Sample results from patient UM56. Black = no template control, green = negative control (wild type),
blue = positive control (primary uveal melanoma cells), orange = metastatic uveal melanoma cells
Session IV: Nanosafety research in regulatory context: Challenges and opportunities
Preparation of solutions for the comet assay and insertion of the samples into the electrophoretic solution
.....at the end.