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Training in advanced in vitro models (Slovakia)


Partner Topic of the training Training description Proposed duration of the training Planned date
BMC SAV Training in advanced in vitro models Preparation of different types of 3D in vitro models (spheroids, multicellular aggregates). Real time evaluation in proliferation, cell death, toxicity, invasiveness of tumour cells using IncuCyte ZOOM™ Imaging system. Stable genetic modification of model cells by retroviral vectors. 1-2 persons / partner for 1 month November 2021


Cell lines have been an irreplaceable tool in cancer research. Many studies have been performed on adherent (2D) in vitro cultures which possess many limitations including affected cell-cell and cell-microenvironment interactions, changes in cell morphology and polarity, the absence of nutrient and oxygen gradient. Three dimensional (3D, spheroid) cultures have overcome some of the mentioned limitations, but they do not recapitulate the heterogeneity of the original tumour.
Tumour organoids recently represent the most relevant in vitro model for the study of tumour biology and testing of therapeutic regimens. These self-organizing and self-renewing microtissues recapitulate the characteristic of original tumour tissue such as complex organization, tissue-specific functions, and phenotype.
The aim of the course is to acquaint the participants with the preparation of 3D multicellular aggregates and their use in the assessment of treatment efficacy. The second part will be focused on the preparation of organoids from colorectal cancer patient-derived xenografts and training the method of tissue dissociation, organoids plating and sub-culturing.