ENG flag

TRAININGS

 

Training in epigenetic analyses – DNA methylation
(Slovakia)

 

Partner Topic of the training Training description Proposed duration of the training Planned date
BMC SAV Course in epigenetic analyses – DNA methylation DNA methylation analysis by pyrosequencing (DNA extraction, Bisulfite modification, PCR, pyrosequencing, data analysis and interpretation) Up to 5 students for 1 week Open for applications from January 2022

 

DNA methylation is one of the key epigenetic mechanisms which plays a significant role in the regulation of gene expression. Is associated with a number of key processes including genomic imprinting, X-chromosome inactivation, repression of transposable elements. Aberrant epigenomic landscapes have been implicated in ageing, autoimmune diseases as well as carcinogenesis. Quantitative analysis of DNA CpG methylation helps to elucidate its role in clinical pathologies. Pyrosequencing is a method based on a sequencing-by-synthesis approach originally designed to quantify single-nucleotide polymorphisms. Bisulfite modification permits utilization of this method for measurement of DNA methylation locally and globally in real-time. The methylation pattern of DNA has the potential to serve as an early detection marker and a potential drug target. The course “Training in epigenetic analyses – DNA methylation” will allow participants to understand and perform all steps of DNA methylation analysis, including DNA extraction, bisulfite modification, PCR amplification and pyrosequencing reaction. Importantly, also bioinformatic analyses, primer design and data analysis are presented and trained.